Aasian dating 144 txt 144
The original PARK6 family (the Marsala kindred) and two other families, which this group has previously mapped to 1p36–35 by linkage analysis, were chosen for analysis by direct sequencing, to determine whether PARK6 was caused by D-1 mutations.
DNA was extracted from peripheral lymphocytes according to standard protocols.
there has been much interest in ascertaining its biological and clinical importance.
To date, however, there have been no published data estimating DJ-1 prevalence in Parkinson’s disease.
A semiquantitative multiplex polymerase chain reaction (PCR) was used to detect exon rearrangements (deletions and duplications).
Only patients with a single heterozygous mutation were included in this heterozygous parkin subgroup.
Two patients reported depression, but none suffered anxiety disorder, which has also been noted in DJ-1.
The hypothesis was also tested that young onset Parkinson’s disease patients in whom, despite extensive analysis, only a single heterozygous parkin mutation was found, might harbour a second mutation in the DJ-1 gene—that is, digenic inheritance.This has led to suggestions that a single mutation in these patients may be sufficient to cause the phenotype, either by haplo-insufficiency or by a dominant negative effect.An alternative hypothesis that we chose to test is that these patients may also harbour pathogenic mutations in the DJ-1 gene and that only the combination of a heterozygous mutation in each of these genes resulted in Parkinson’s disease.including PARK6 (1p36–p35), which appears to have a broadly similar phenotype.This has led to suggestions that PARK6 and PARK7 are allelic, either because of chromosomal rearrangements or because of erroneous linkage mapping.